Molecular and Cellular Biologist - DAF Functional Genomics
Our client, a world-leading Pharmaceutical Company in Cambridge, MA is currently looking for a Molecular and Cellular Biologist to join their expanding team.
Job Title: Molecular and Cellular Biologist
Duration: 6 months contract, extendable up to 36 months
Location: Cambridge, MA
Note:
The client has the right-to-hire you as a permanent employee at any time during or after the end of the contract.
You may participate in the company group medical insurance plan
Job Description: (Required):
Molecular and Cellular Biologist DAF Functional Genomics
Summary:
We are seeking a Molecular and Cellular Biologist DAF Functional Genomics to join our Systems Immunology Lab in Pfizer's Inflammation & Immunology Research Unit.
The successful candidate will be responsible for generating massive single-cell perturbational data using functional genomics and single-cell transcriptomics technologies in collaboration with a small team.
Responsibilities:
Generate custom lentiviral libraries and maintain extensive cell cultures
Generate edited pools of cells via lentiviral transduction and arrayed electroporation
Generate single-cell data via several commercial workflows, including those from 10x Genomics
Develop, modify, and customize such procedures as needed to succeed in specific cell lines and types, and at large scale
Qualifications:
Bachelor's or Master's and a minimum of 3+ years of experience in Biology, Immunology or a related field
A minimum of 3+ years of hands-on experience with standard cell culture techniques, including primary immune cells handling and isolation, maintenance of cell lines, and in vitro stimulation of primary immune cells
Experience with standard molecular biology techniques, including lentivirus infection, qPCR, western blot, and flow cytometry
Experience with diverse cell types
Direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing preferred
Location/Schedule:
Fully onsite role in Cambridge, MA
Monday - Friday; 40 hours per week
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