Research Associate/Senior Research Associate, In Vivo

Thymmune Therapeutics
Cambridge, MA
About Us

Thymmune Therapeutics is a preclinical biotechnology company based in Cambridge, MA applying proprietary insights in thymic engineering to develop and commercialize immune cell therapy products addressing significant unmet clinical need across immunology. Building on work from leading labs, Thymmune has strong IP positioning and is backed by early investors and founders of successful high-profile biotechnology companies.

Role Description

We are seeking a highly motivated and technically skilled Research Associate to join our rapidly growing team. This role sits at the intersection of in vivo pharmacology and Immunology within the R&D department. You will be responsible for testing the functionality, safety, and efficacy of iPSC-derived thymic epithelial cell therapies in mouse models.

The ideal candidate possesses an industrial mindset underpinned by critical thinking and the ability to multitask in a fast-paced environment. You will embody our "Always Learning" value, collaborating across teams to deepen our collective knowledge. You will not only execute complex studies but also participate in strategic planning, data analysis, and the mentorship of junior staff.

Key Responsibilities

In vivo Studies and Pharmacology
  • Execute in vivo studies using mouse models, including dosing, monitoring, and tissue collection at necropsy.
  • Perform standard dosing and sampling techniques, including SC, IM, IV (retro-orbital), and IP injections, as well as submandibular bleeds and tail nick.
  • Conduct or assist with small rodent surgical procedures (e.g., tissue transplantation/implantation); experience in this area is a key asset.
  • Perform specialized tissue processing and imaging techniques, including immunohistochemistry (IHC) and immunofluorescence (IF) staining.
  • Assess in vivo functionality, safety, and tumorigenicity of stem cell-derived cell product.
Cell culture & In Vitro biological assays
  • Maintain and expand primary cell cultures using aseptic technique
  • Perform cell isolation from primary human or mouse tissues for downstream analysis
  • Conduct downstream biological assays to validate cell quality and function, including Flow Cytometry (FACS), RT-qPCR, and ddPCR, MSD/ELISA.
  • Analyze biomarkers and signalling pathways to correlate in vitro characteristics with in vivo outcomes.
Data Analysis, Reporting, & Collaboration
  • Analyze complex datasets using statistical software (e.g., GraphPad Prism) and present findings to the scientific team.
  • Maintain high standards of data integrity and ensure accurate documentation for research reports and study protocols.
  • Contribute to the writing of study reports and participate in the strategic planning of long-term studies.
  • Collaborate with cross-functional teams, support junior laboratory staff and interns, fostering a collaborative working environment.
Our Values

At Thymmune, our culture is the foundation of our success. We are looking for a candidate who embodies our core principles:
  • Human Connection : We respect and care for each other, our patients, and our communities. We believe teamwork empowers our joint success.
  • Pioneering Spirit : We are trailblazers. We fearlessly push boundaries to overcome the limitations of existing therapies, fueled by passion and cutting-edge science.
  • Always Learning : We believe collaboration drives innovation. Curiosity deepens our knowledge, and we benefit from our collective ideas and perspectives.
  • Delivering on Our Promise : We are accountable to each other and the people we serve. We make a difference by delivering on our vision and elevating one another.
Required Qualifications and Skills

  • Education: B.S. or M.S. in Cell Biology, Immunology, Regenerative Medicine, or a related discipline.
  • Experience: 3–5+ years of relevant industry experience (Biotech/Pharma experience preferred).
  • In Vivo Skills: Proficiency in rodent handling, multiple routes of administration (IV, IP, SC), and tissue dissection is required.
  • In Vitro Skills: Hands-on experience with the isolation and culture of primary human cells or tissues is preferred.
  • Analytical Skills: Strong experience with Flow Cytometry, qPCR, and microscopy. Ability to analyze data statistically and troubleshoot experimental variances independently.
  • Other Skills: Excellent oral and written communication skills. Must be able to structure tasks, set priorities, and work effectively in a collaborative, team-oriented environment.

Posted 2026-01-30

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