Scientist I

Overview

The Targeted Protein Degradation (TPD) Proteomics Core within the Fischer Lab at Dana-Farber Cancer Institute serves as a resource for the Cancer Biology Department, aiding investigators in answering key research questions using mass spectrometry-based proteomics. We employ quantitative chemoproteomic and interactomic approaches to identify drug targets and are continuing to expand our repertoire to meet the needs of our core users. Our core is equipped with extensive mass spectrometry equipment including timsTOF HT, timsTOF Ultra2 and Orbitrap Eclipse tribrid mass spectrometers.

The TPD Core seeks a highly motivated individual to join the team as a Research Scientist I, who will employ mass spectrometry-based technologies to assess the cellular proteome in response to external stimuli. This individual will be an important member of our core team and will work with technicians, scientists, postdocs and students on a variety of different research projects focused on targeted protein degradation as a novel therapeutic modality. While we consider various mass spectrometry related backgrounds, a strong interest and understanding of mass spectrometry-based proteomics is a must and strong computational skills are highly desirable.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world-renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

Responsibilities

• Lead the design and execution of proteomics experiments (eg, TMT, DIA, DDA) in collaboration with internal and external investigators
• Develop, optimize, and troubleshoot sample preparation workflows including whole cell proteomics, interactome analysis, and various enrichment protocols
• Performs operation, maintenance and data acquisition on high-resolution mass spectrometers (eg. Eclipse, timsTOF)
• Analyze data using advanced pipelines (eg, DIA-NN, spectronaut, proteome discoverer) and support biological interpretation.
• Develop and validate new methods as required
• Train and mentor junior staff and contribute to a culture of continuous improvement
• Ensure compliance with lab safety, QC standards, and data integrity policies
• Maintains highly organized sample and data tracking and reporting workflows
• Communicates with purchasing and may be responsible for negotiating with vendors

Qualifications

• Ph.D in biochemistry, analytical chemistry, cancer biology or related field required
• Strong expertise in mass spectrometry-based proteomics and data analysis
• Experience operating Thermo and/or Bruker mass spectrometers is required
• Experience with quantitative proteomics (label-free, TMT, DIA) and enrichment techniques.
• Proficiency with data analysis software (eg. DIA-NN, Proteome Discoverer, R, Python)
• Ability to work collaboratively with interdisciplinary teams and external clients.
• Demonstrated track record of scientific productivity (publications, presentations)
• Excellent organizational and communication skills

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law .