Research Technician (Polizzi Lab)

Overview

The Polizzi lab uses a mix of computational and experimental approaches to design and characterize ligand-binding proteins. We develop machine-learning techniques to compute the structures and sequences of proteins that bind to challenging small-molecule ligands. We then express these proteins in the lab and characterize them with biophysical and structural methods (see, e.g., Ashworth, Polizzi & DeGrado, Science 2024). We aim not only to unlock the design principles used by proteins for molecular recognition but also to make useful proteins for probing and manipulating biology. By designing de novo small-molecule-binding proteins, we can isolate the essential ingredients for protein molecular recognition events, obtain atomic-level control of the protein/small-molecule interface, and set the stage for the development of proteins that could serve as novel sensors, enzymes, drug delivery vehicles, or pharmaceuticals.

A research technician position is available in the lab to contribute to experimental pipelines for the characterization of designed small-molecule binding proteins. This entry-level position is a great opportunity to gain experience working in a cutting-edge protein design laboratory. We are seeking enthusiastic individuals who possess strong interpersonal skills, have a growth mindset, are highly organized, and who interact well with others as part of a collaborative team.

The ideal candidate will have laboratory experience in protein biochemistry (protein expression and purification using E. coli, yeast, or mammalian cell systems, as well as basic molecular biology techniques) or a willingness to learn. Some experience in structural methods (X-ray crystallography, electron microscopy, or NMR) and/or protein-display methods (e.g., yeast-, phage-, or ribosome-display) would be desirable but is not necessary. While this role is predominantly focused on performing wet-lab experiments, there is also ample opportunity for the candidate to learn some computational protein design skills while on the job.

Candidates with a background that encompasses both dry- and wet-lab experience are also encouraged to apply.

Our lab at Dana-Farber’s Longwood Center has access to state-of-the-art facilities, including for cryo-EM, X-ray crystallography, flow cytometry, and high-performance computing. We are part of a highly collaborative, interdisciplinary environment within the Chemical Biology program at the Dana-Farber Cancer Institute and the Department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School. We collaborate with leading biologists and physician scientists to pursue high impact applications of protein design for understanding and controlling biology.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS, and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

• Protein expression and purification

• Cloning and mutagenesis

• Flow cytometry and yeast protein surface display

• Assist in structure determination by cryo-EM and/or X-ray crystallography

• Perform binding studies using SPR, BLI, fluorescence polarization, calorimetry, etc.

• Attend team meetings to share results

• Document findings in a well-managed lab notebook, and oral/written reports.

• Set up, adjust, calibrate, clean, maintain and troubleshoot equipment

• Clean, maintain and prepare supplies and work areas and may be responsible for ordering of supplies. Collaborates with Purchasing and may be responsible for negotiating with vendors

• Conduct standardized biological, microbiological or biochemical tests and laboratory analyses, including the collection of information and samples from organic substances, such as blood, drugs, and animals

• Isolate, identify and prepare specimens for examination

• Monitors laboratory work to ensure compliance with Environmental Health and Safety information as well as the standard operation procedures specific to each laboratory

• Expected to provide intellectual and interactive commitment to position by keeping up with appropriate literature and research publications

• May be responsible for the development, modification and improvement of standard operating procedures

• Uses computers, computer-interfaced equipment, robotics or high-technology applications to perform work duties

• May be responsible for designing and executing advanced experiments and setting strategy

KNOWLEDGE, SKILLS, AND ABILITIES REQUIRED:

• Knowledge of information and techniques related to either biology, chemistry, or medicine is preferred

• Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff

• Ability to analyze information and use logic to address work-related issues and problems

• Must have attention to detail and be thorough in completing work tasks

• Ability to work within a team environment

MINIMUM JOB QUALIFICATIONS:

The position requires a bachelor’s degree, preferably in biology, biophysics, biochemistry, computer science, or related fields, with no prior professional experience necessary. We are looking for candidates with at least 2 years of availability.

SUPERVISORY RESPONSIBILITIES:

No supervisory responsibilities. Normally receives detailed instructions on all work.

PATIENT CONTACT: None

At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong. As relentless as we are in our mission to reduce the burden of cancer for all, we are committed to having faculty and staff who offer multifaceted experiences. Cancer knows no boundaries and when it comes to hiring the most dedicated and compassionate professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other characteristics protected by law.

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Pay Transparency Statement

The hiring range is based on market pay structures, with individual salaries determined by factors such as business needs, market conditions, internal equity, and based on the candidate’s relevant experience, skills and qualifications.

For union positions, the pay range is determined by the Collective Bargaining Agreement (CBA).

$47,500.00 - $51,300.00